Plasmid_Backbone

Part:BBa_K1085014:Design

Designed by: S. P. van Kessel   Group: iGEM13_Groningen   (2013-09-11)


pSB1AC3-HySp: Integrational backbone into B. subtilis amyE locus with IPTG inducible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 7604
    Illegal NheI site found at 7428
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 7610
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 7604
    Illegal BamHI site found at 6066
    Illegal XhoI site found at 3574
    Illegal XhoI site found at 7488
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 7604
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 7604
    Plasmid lacks a suffix.
    Illegal XbaI site found at 7619
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1128
    Illegal NgoMIV site found at 3538
    Illegal NgoMIV site found at 4677
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 4501


Design Notes

In pDR111 the Hy_spank promoter and the lacI gene are orientated in the same direction. In order to have the Hy_spank promoter right in front of the prefix we turend the promoter around.


Source

iGEM Munich 2012 (Rudner Lab 2004) and Rudner's Lab 2004 (pDR111)

References